Study macrophage/ monocyte brain invasion, morphology and motility and interaction with brain parenchymal cells by intravital 2P microscopy

Examine the recruitment and activation of macrophages/ monocytes and other immune cell sets by flow cytometry and rtPCR

Characterize macrophage/ monocyte differentiation by single-cell transcriptomics

Evaluate the effect of macrophage/ monocyte depletion and deactivation on ischemic injury and stroke recovery

Evaluate the effect of targeted macrophage/ monocyte transdifferentiation followed by adoptive cell transfer on ischemic injury and stroke recovery

Examine the effect of MSC-EVs in combination with physical exercise, nutrition modification or monocyte/ macrophage preconditioning on ischemic injury and stroke recovery