PHASE I - Study Of MDSCs Role In Severe Bacterial And Fungal Infections. Biobank Design ( 01.01.2020-30.04.2020 )
-generating data relating to the modification of the transcriptome of MDSCs induced severe infections and their comparison with data obtained from other populations of immune cells
-Storage of biological samples
-Data analysis in order to communicate in scientific events and their publication in journals.
A1.1 Literature review
A1.2 SOPs, protocols, CRFs development
A1.3 Website setup. Database and biobank design
PHASE II – Study Of MDSCs Isolated From Patients Diagnosed With Sepsis And Healthy Volunteers ( 01.01.2019-31.12.2019 )
Study MDSCs isolated from patients diagnosed with sepsis and healthy volunteers.Ongoing pilot study of the project.
1.Line Configuration cell isolation using magnetic particles
2.Ongoing pilot study of the project.
3.Informed consent, data collection (history, clinical examination)Harvesting, processing and evidence .Archiving
4.MDSCs in vitro study
5.Functional Validation of the results obtained in the experiments carried out in vitro.
PHASE III – Assessment Of Interaction Between MDSCs, Infectious Agents And Host Immunity ( 09.05.2018-31.12.2018 )
Analysis of the interaction between MDSCs, infectious agents and human immunity
PHASE III – Assessment of interaction between MDSCs, infectious agents and host immunity