BISTAR

Etape

 

Phase 1 (2015) ( 3 months )

Obiective

1.1.Setting up project management
1.2.Initiating testing and optimizations

Activitati

1.1.a. Work meeting between partners; establishing the final working protocols
1.1.b. Signing an agreement with the Ethics Committee of UMF Craiova; creating a dedicated web page

1.2.a. Initiating testing and optimization of iDISCO technique (IHC enabled three dimensional imaging of solvent-cleared organs).
1.2.b. Acquisition of equipment and ancillaries.
1.2.c. Submitting the scientific summary

Phase 2 (2016) ( 12 months )

Obiective

2.1. Continuous testing of the working protocols, set up of the animal control group
2.2. Set up and analysis of the OACM/GLT-1 animal group
2.3. Set up and analysis of the OACM/AQP4 animal group
2.4. Set up and analysis of the OACM/AQP4-GLT-1 animal group
2.5. Initiation of integrative pathological analysis

Activitati

2.1.a. Establishment of the final iDISCO protocol.
2.1.b. Thorough testing of the immunohistochemistry protocols needed during the project
2.1.c. Establishment of the animal control groups: (i) OACM without treatment, (ii) “sham” simulation group, and (iii) intervention-free group
2.1.d. Patent application
2.1.e. Behavioural evaluation of the animals; archiving harvested tissue and initiation of immunohistochemistry and iDISCO studies
2.1.f. Acquisition of equipment and ancillaries.

2.2.a. Set up of the OACM animal group treated with GLT-1 activator
2.2.b. Behavioural evaluation of the animals from OACM/GLT-1 group; archiving harvested tissue and continuation of immunohistochemistry iDISCO studies


2.3.a. Set up of the OACM animal group treated with AQP4 inhibitor
2.3.b. Behavioural evaluation of the animals from OACM/ AQP4 group; archiving harvested tissue and continuation of immunohistochemistry iDISCO studies

2.4. a. Set up of the OACM animal group treated with AQP4 inhibitor and GLT-1 activator
2.4. b. Behavioural evaluation of the animals from OACM/AQP4-GLT-1 group; archiving harvested tissue and continuation of immunohistochemistry iDISCO studies

2.5. a. Pathological, immunohistochemical and integrative iDISCO analysis
2.5. b. Submitting the scientific summary

Phase 3 (2017) ( 9 months )

Obiective

3.1. Continuous integrative pathological analysis
3.2. Finalising the analysis of the OACM/AQP4-GLT-1 animals group
3.3. Finalising the integrative pathological analysis
3.4. Final global analysis

Activitati

3.1. a. Laser microdissection and quantitative analysis

3.2.a. Continuous behavioural evaluation of the animals from OACM/AQP4-GLT-1group; archiving harvested tissue and continuation of immunohistochemistry iDISCO studies
3.2.b. Acquisition of equipment and ancillaries.


3.3.a. Finalising pathological, immunohistochemical and iDISCO-related analysis
3.3.b. Finalising quantitative analysis

3.4.a. Integrative analysis of behavioural, pathological and volumetrical data
3.4.b. Final work meeting and submission of the final scientific report